InCyt Basic PMTM                                                                                        (click image to enlarge and get detailed specs)

Complete Turnkey System

The InCyt Basic PmTM Photometry System includes everything needed for calcium and other single excitation and ratiometric fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, photon counting photometer assembly, computer controlled filter changer, Mercury-Xenon  lamp, data acquisition & analysis computer workstation and data acquisition/analysis software.

Ease-of-use

The InCyt Basic PmTM Photometry System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.

 

Data Collection, Analysis & Presentation

Data can be measured continuously in user definable regions of interest. The results are displayed in real time and can be saved for post hoc analysis. Ion concentration within cells or ratio values can be displayed. Data files generated by InCyt Basic PmTM are stored as ASCII text.

 

Specifications

Photometer Module
PMTHamamatsu HC135-11 photon counter
Linearity (precision)+/-1% from 0 to 20,000,000 counts/second
Maximum Dark Count (noise)150 counts/second
Equivalent Noise Input (noise)3 x 10-17 watts per second (measured at 400nm)
Spectral Sensitivity300-650nm
Discriminator & MicroprocessorBuilt into PMT
Overload ProtectionComputer-controlled shutter (optional)
Excitation Light Source250 watt Xenon arc lamp for both UV and visible light excitation. Variable intensity
Filter ChangerTofra Wheel Filter Changer


Data Acquisition and Analysis Workstation
CPUIntel®  CoreTM  2 Duo Processor E4600 (2.4GHz, 2M, 800MHz FSB)
RAM1GB SDRAM,667MHz
Hard Drive80GB SATA 3.0Gb/s and 8MB DataBurst Cache
DVD ROM16X DVD +/- R/W
Operating SystemWindows XP
Monitor17" SVGA


Data Capture and Display
Maximum Speed6 ratiometric points per second
100 single-wavelength points per second
Time LapseUp to 1 measurement every 10 minutes
Field of ViewRectangular area, user-definable
Graphic DisplayReal-time during experiment
Data Storage FormatTab-delimited ASCII


Other
CalibrationFrom standard solutions, formula, or in situ
IonsWorks with hundreds of fluorescent dyes for measuring: Ca2+, pH, Na+, K+, Mg+2, H2O2, cyclic ADP Ribose, etc.

 

 

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