Intracellular Imaging, Inc. - InCyt Im2 Imaging System

InCyt High Speed IMTM                                                                (click image to enlarge and get detailed specs)

Complete Turnkey System

 The InCyt High Speed ImTM Imaging System includes everything needed for calcium and other single excitation and ratiometric fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, low-light level integrating CCD camera, computer controlled fast changing monochromator Xenon arc lamp, image processing computer and data acquisition/analysis software.

Ease-of-use

The InCyt High Speed ImTM Imaging System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.

 

Data Collection, Analysis & Presentation

Data can be measured continuously on as many as 50 pre-selected regions of interest. The results can displayed in real time or images can be saved for post hoc analysis. Saved images can be played back as an animated sequence—ideal for analysis of sub-cellular responses, intercellular communication and heterogeneity among the individual cells in a population. Ion concentration within cells can be converted to color using standard or custom pseudo-color tables. Image sequences can be presented as a montage with user-defined annotation for presentation and publication. Data generated by InCyt High Speed ImTM are stored in a standard TIFF format and data files are stored as ASCII text.


Specifications

Camera Module
Camera DVC 340M Monochrome Integrating 12-bit CCD with Binning Capabilities
Excitation Wavelength Changer Till Polychrome V fast changing monochromator with Xenon arc lamp
Image Resolution 640 x 480 pixels


Image Acquisition and Data Analysis Workstation
CPU Intel®  CoreTM  2 Duo Processor E4600 (2.4GHz, 2M, 800MHz FSB)
RAM 2GB SDRAM,667MHz
Hard Drives 80GB/160GB SATA 3.0Gb/s and 8MB DataBurst Cache
DVD ROM 16X DVD +/- R/W
Operating System Windows XP
Monitor 17" SVGA


Data Capture and Display
Maximum Speed 20 ratiometric points per second
140 single-wavelength points per second
Object Definition Specify up to 50 user-defined regions for separate analysis
Data Collection Options (1) Graph ion concentration in each cell during experiment.
(2) Save images for later analysis and animated playback
Image Format TIFF
Data Storage Format Tab-delimited ASCII


Other
Calibration From standard solutions or formula
Ions Works with hundreds of fluorescent dyes for measuring: Ca2+, pH, Na+, K+, Mg+2, H2O2, cyclic ADP Ribose, etc.


Data Presentation