InCyt High Speed PMTM (click image to enlarge and get detailed specs)
Complete Turnkey System
The InCyt High Speed PmTM Photometry System includes everything needed for calcium and other single excitation and ratiometric fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, photon counting photometer assembly, computer controlled fast changing monochromator Xenon arc lamp, data acquisition & analysis computer workstation and data acquisition/analysis software.
Ease-of-use
The InCyt High Speed PmTM Photometry System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.
Data Collection, Analysis & Presentation
Data can be measured continuously in user definable regions of interest. The results are displayed in real time and can be saved for post hoc analysis. Ion concentration within cells or ratio values can be displayed. Data files generated by InCyt High Speed PmTM are stored as ASCII text.
Specifications
| PMT | Hamamatsu H7360-01 photon counter |
| Linearity (precision) | +/-1% from 0 to 20,000,000 counts/second |
| Maximum Dark Count (noise) | 150 counts/second |
| Equivalent Noise Input (noise) | 3 x 10-17 watts per second (measured at 400nm) |
| Spectral Sensitivity | 300-650nm |
| Discriminator & Microprocessor | Built into PMT |
| Overload Protection | Computer-controlled shutter (optional) |
| Excitation Wavelength Changer | Till Polychrome V fast changing monochromator with Xenon arc lamp |
| CPU | Intel® CoreTM 2 Duo Processor E4600 (2.4GHz, 2M, 800MHz FSB) |
| RAM | 1GB SDRAM,667MHz |
| Hard Drive | 80GB SATA 3.0Gb/s and 8MB DataBurst Cache |
| DVD ROM | 16X DVD +/- R/W |
| Operating System | Windows XP |
| Monitor | 17" SVGA |
| Maximum Speed | 150 ratiometric measurements per second 1000 single-wavelength measurements per second |
| Time Lapse | Up to 1 measurement every 10 minutes |
| Field of View | Rectangular area, user-definable |
| Graphic Display | Real-time during experiment |
| Data Storage Format | Tab-delimited ASCII |
| Calibration | From standard solutions, formula, or in situ |
| Ions | Works with hundreds of fluorescent dyes for measuring: Ca2+, pH, Na+, K+, Mg+2, H2O2, cyclic ADP Ribose, etc. |