Products > PM Systems > Basic PM System

Complete Turnkey System

The InCyt Basic PmTM Imaging System includes everything needed for calcium and other single excitation and ratiometric fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, low-light level integrating CCD camera, computer controlled filter changer, Xenon UV/VIS arc lamp, image processing computer and data acquisition/analysis software.


The InCyt Basic PmTM Imaging System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.

Data Collection, Analysis & Presentation

Data can be measured continuously on as many as 50 pre-selected regions of interest. The results can displayed in real time or images can be saved for post hoc analysis. Saved images can be played back as an animated sequence—ideal for analysis of sub-cellular responses, intercellular communication and heterogeneity among the individual cells in a population. Ion concentration within cells can be converted to color using standard or custom pseudo-color tables. Image sequences can be presented as a montage with user-defined annotation for presentation and publication. Data generated by InCyt Basic PmTM are stored in a standard TIFF format and data files are stored as ASCII text.


Photometer Module


Hamamatsu HC135-11 photon counter

Linearity (precision)

+/-1% from 0 to 20,000,000 counts/second

Maximum Dark Count (noise) 150 counts/second
Equivalent Noise Input (noise) 3 x 10-17 watts per second (measured at 400nm)
Spectral Sensitivity 300-650nm
Discriminator & Microprocessor Built into PMT
Overload Protection Computer-controlled shutter (optional)
Excitation Light Source Sutter Instruments LB-LS17 light source with 175W Xenon arc
lamp for both UV and visible light excitation.
Filter Changer Tofra Wheel Filter Changer

Data Acquisition and Data Analysis Workstation

Intel®  CoreTM  2 Duo Processor E4600 (2.4GHz, 2M,

800MHz FSB)

Hard Drives 250GB SATA 3.5" SATA 6Gb/s with 8MB DataBurst Cache
Operating System Windows XP
Monitor 17" SVGA

Data Capture and Display
Maximum Speed 6 ratiometric points per second
100 single-wavelength points per second
Time Lapse Up to 1 measurement every 10 minutes
Field of View Rectangular area, user-definable
Graphic Display Real-time during experiment
Data Storage Format Tab-delimited ASCII

Calibration From standard solutions or formula

Works with hundreds of fluorescent dyes for measuring:

Ca2+, pH, Na+, K+, Mg+2, H2O2, cyclic ADP Ribose, etc.