Intracellular Imaging, Inc. - Application Notes, Fluorophores, Fura-2, Fluo-3, Quin-2, Mag-Fura-2

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Common Fluorophores

**Single Excitation - Single Emission Dyes**
ION	PROBE	Ex Wavelength (nm)	Em Wavelength (nm)
Calcium	Quin-2	339	400
Calcium	Fluo-3	488	530
Calcium	Fura-Red	488	660
Calcium	Calcium Green	488	530
Calcium	Calcium Orange	550	580
Calcium	Calcium Crimson	593	615
Calcium	Rhod-2	550	575
Chloride	SPQ	320	440
Chloride	SPA	355	490
Chloride	MQAE	350	460

**Dual Excitation - Single Emission Dyes**
ION	PROBE	Ex 1 Wavelength (nm)	Ex 2 Wavelength (nm)	Em Wavelength (nm)
Calcium	Fura-2	340	380	510
Magnesium	Mag-Fura-2	340	380	510
Magnesium	Mag-Fura-5	340	380	500
Memb. Po.	Di-4-ANEPPS	475		617
Memb. Po.	Di-8-ANEPPS	475		605
pH	BCECF	440	490	530
pH	SNAFL-1	514	550	600
Sodium	SBFI	340	380	505
Potassium	SBFI	340	380	505